Biochemistry of homologous recombination in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Biochemistry of homologous recombination in Escherichia coli.
Homologous recombination is a fundamental biological process. Biochemical understanding of this process is most advanced for Escherichia coli. At least 25 gene products are involved in promoting genetic exchange. At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA pol...
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The biochemistry of eukaryotic homologous recombination caught fire with the discovery that Rad51 is the eukaryotic homolog of the bacterial RecA and T4 UvsX proteins; and this field is still hot. The core reaction of homologous recombination, homology search and DNA strand invasion, along with the proteins catalyzing it, are conserved throughout evolution in principle. However, the increased c...
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Recombination between two different deletion alleles of a gene (neo) for neomycin and kanamycin resistance was studied in an Escherichia coli sbcA- recB-C- strain. The two homologous regions were in an inverted orientation on the same plasmid molecule. Kanamycin-resistant plasmids were selected and analyzed. The rate of recombination to form kanamycin-resistant plasmids was decreased by mutatio...
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Methylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and methyl methane sulfonate (MMS) produce a wide variety of N- and O-methylated bases in DNA, some of which can block replication fork progression. Homologous recombination is a mechanism by which chromosome replication can proceed despite the presence of lesions. The two major recombination pathways, RecBCD and RecFOR, whi...
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AKALYSIS of mixed cultures. sf nutritional mutants has revctlled the presence of new types which strongiy suggest the occurrence of a sexual process in the bacterium; EsciumcJ~ia coti. The mutants consist of strains which differ from their parent wild type, strain I.-l’& in lacking the ahilitv to synthesize growth-factors. . As a result of these dejiciencies they <ill only grow in media supplem...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1994
ISSN: 0146-0749
DOI: 10.1128/mr.58.3.401-465.1994